Description fugene 6 transfection reagenta is a nonliposomal reagent that transfects dna into a wide variety of cell lines with high e. Excerpts and links may be used, provided that full and clear credit is given to promega corporation with appropriate and specific direction to the original content. We found that the fugene 6 and fugene hd reagents perform as well. Nanobret a novel bret platform for the analysis of proteinprotein interactions thomas machleidt1, carolyn c. Transfection is the process of deliberately introducing naked or purified nucleic acids into eukaryotic cells. Adherent cells can be trypsinized and transfected by the dna. It may also refer to other methods and cell types, although other terms are often preferred. Fugene hd transfection reagent quick protocol fb112pdf 99 kb english.
The fugene hd transfection reagent is a nonliposomal formulation designed to transfect dna into a wide variety of cell lines with high efficiency and low toxicity. Fugene hd transfection reagent lets you work in your cancer model. First, not every transfection reagent is perfect for every cell line. Fugene hd is sold with a limited use label license lull. Fugene promega e2311 is a nonliposomal transfection reagent designed to work, with high efficiency and low toxicity, in a wide variety of cell lines. During development of each reagent, good transfection efficiency was verified in a variety of cells as indicated in the product manual. Our teams are in regular contact with suppliers and distributors worldwide and are taking all steps necessary to address both demands for diagnostic tools and reliable delivery of all products as quickly as possible. To be the most responsive supplier of biological reagents and reagent systems used in research and applied technology applications worldwide. Incubate at room temperature for 20 minutes to form complexes. Fugene hd transfection reagent for efficient mammalian. Vortex and incubate the mixture at room temperature for. Always mix fugene 6 reagent prior to use vortex for one second or use inversion. Fugener hd transfection reagent technical manual tm328.
Fugene 6 transfection reagent is a nonliposomal formulation designed to transfect plasmid dna into a wide variety of cell lines with high efficiency and low toxicity. Use the fugene hd protocol database to see if transfection conditions for your cell line are available. Promega manufacturing and delivery systems continue to be fully operational during the covid19 outbreak. Helen sarantis, sergio grinstein, in methods in cell biology, 2012. Hi guys i am a new researcher, i try to do transfection in hek 293 cells using fugene hd transfection reagent but failed, i am very confused now what should i doif somebody have some knowledege. Additionally, low cell numbers can be transfected in 96well plates.
Each promega product is shipped with documentation stating specifications and other technical information. With fugene hd, there is no need to change media after an. Citation list of established cells transfected with fugene. In animal cells, transfection is the preferred term as.
Lentiviral vectors lvv have become a valuable research tool for delivering genetic content into a wide range of cell types. Nanobret a novel bret platform for the analysis of protein. Fugene 6 reagent complex prior to plating, making it a strong candidate for high throughput applications. Fugene hd is a novel, nonliposomal reagent for transfecting dna into a variety of cell lines with high efficiency and low toxicity. All three offer broad spectrum transfection capability and low. Fugene hd transfection reagent manual active motif. In this report we compare luciferase expression and viability in three cell lines that we transfected using four different transfection reagents, the promega fugene 6 and fugene hd transfection reagents, transitlt1 transfection reagent mirus bio llc and lipofectamine 2000 transfection reagent life technologies. Promega products contain chemicals which may be harmful if misused. Cell suspension and transfection mix were mixed at a ratio of 20.
Before use, allow the vial of fugene hd transfection reagent to reach room. Promega fugene hd transfection reagent fugene hd is a novel, nonliposomal reagent for transfecting dna into a variety of cell lines with high efficiency and low toxicity. These protocols were developed by promega corporation or fugent, l. The database includes over 25 cancer models and provides transfection protocols specific for each cell line. Fugene hd transfection reagent offers unrivaled transfection performance with minimal impact on cell physiology jacobson et al. Optimization of mouse embryonic stem cell transfection for. Viafect transfection reagent transfection assay e4981. This plate reading luminometer is optimized with for use with promega cell and gene reporter assays and comes with over 30 preloaded assay protocols. The day before transfection plate adherent cells in the amount that by the time of transfection.
Fugene hd transfection reagent is a nonliposomal formulation designed. It is suitable for use in media with or without serum, and for transient or stable transfection, as well as cotransfections of multiple dna plasmids. Combinational use of lipidbased reagents for efficient. Fugene 6 get more out of your lentiviral production. Addition ally, the fugene hd transfection reagent has been shown to support transfection in chemically defined media and. Promega corporation is a worldwide leader in applying biochemistry and molecular biology to the development of innovative, highvalue products for the life sciences. Biorad, hercules, ca, and fibro blast transfection reagent altogen. Promega products are warranted to meet or exceed the stated specifications. Invitrogen, carlsbad, ca, usa or fugene hd promega, madison, wi, usa were diluted separately in 50. Fugene hd transfection reagent quick protocol fb112pdf.
Promega corporation materials required hek293, lgbit hek293 stable cell line available through custom assay services or similar cultured mammalian cells. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3 tm328 revised 2 storage conditions. All three offer broad spectrum transfection capability and low toxicity but the same question always comes up. This reagent is suitable for use in media with or without serum, and for transient or stable transfection. Choosing a transfection reagent is easier when you can rely on the work of researchers who have used it before you. It is ideal for use in a variety of challenging applications, including transfection of cancer model cell lines, insect cells, stem cells, and for virus and protein production. Fugene 6 transfection reagent is gentle on the cells. Description a critical key to understanding protein function is the ability to understand a.
Promega offers three premium transfection reagents. The fugene hd protocol database is a collection of protocols that guide the transfection process for a variety of cell lines. Promega corporation 2800 woods hollow road madison, wi 537115399 usa telephone 6082744330 toll free 8003569526 fax 6082772516 internet. Sign up for promega updates and get 15% off your next promega. While considering a suitable transfection reagent, it is. Promega corporation 2800 woods hollow road madison,wi 53711 u. Promega corporation 2800 woods hollow road madison, wi 537115399 usa toll free in usa 8003569526 6082744330 fax 6082772516 3. Fugene hd transfection reagent technical manual promega. Does anyone have any idea what is the main difference between fugene hd transfection reagent and fugene 6 transfection reagent from promega. A protocol for nonliposomal transfection of dna into a variety of cell lines with high efficiency and low toxicity. Delivery of episomal vectors into primary cells by means of commercial transfection reagents. We routinely use fugene hd to introduce plasmids encoding tagged lipidbinding domains into raw264. All offer broad spectrum transfection capability and low toxicity.
Promega fugene 6 transfection reagent fugene 6 transfection reagent is a nonliposomal formulation designed to transfect plasmid dna into a wide. Vwr offers transfection reagents for numerous applications, including bioproduction, in vivo work, viral production, dna and sirna transfection, crisprcas 9 and more. Fugene hd transfection reagent promega corporation. Due care should be exercised with all promega products to prevent direct human contact. The protocol does not require removal of serum or culture medium and does not require washing or changing of medium after introducing the reagentdna complex. Moreover, transfection protocols using lipidbased reagents are simple and quite similar.